| Name | Pos | Birth | Nat |
|---|---|---|---|
| Przemysław Tytoń | GK | 04/01/87 | Poland |
| Lars Unnerstall | GK | 20/07/90 | Germany |
| Issam El Maach | GK | 01/02/00 | Morocco |
| Sam Karssies | GK | 26/02/03 | Netherlands |
| Robin Pröpper | DF | 23/09/93 | Netherlands |
| Stav Lemkin | DF | 02/04/03 | Israel |
| Guilherme Peixoto | DF | 25/05/06 | Portugal |
| Ruud Nijstad | DF | 26/05/08 | Netherlands |
| Marko Pjaca | MF | 06/05/95 | Croatia |
| Thomas van den Belt | MF | 18/06/01 | Netherlands |
| Benjamin Nagel | MF | 11/11/07 | Netherlands |
| Lucas Vennegoor of Hesselink | FW | 14/03/06 | Netherlands |
| Sondre Ørjasæter | FW | 28/11/03 | Norway |
| Daouda Weidmann | Central Midfield | 04/05/03 | France |
| Mats Rots | Left-Back | 11/03/06 | Netherlands |
| Kristian Hlynsson | Attacking Midfield | 23/01/04 | Iceland |
| Max Bruns | Centre-Back | 06/11/02 | Netherlands |
| Daan Rots | Right Winger | 25/07/01 | Netherlands |
| Mees Hilgers | Centre-Back | 13/05/01 | Indonesia |
| Taylor Booth | Right Winger | 31/05/01 | USA |
| Naci Ünüvar | Left Winger | 13/06/03 | Turkey |
| Mathias Kjølø | Defensive Midfield | 27/06/01 | Norway |
| Ramiz Zerrouki | Defensive Midfield | 26/05/98 | Algeria |
| Bart van Rooij | Right-Back | 26/05/01 | Netherlands |
| Ricky van Wolfswinkel | Centre-Forward | 27/01/89 | Netherlands |
| Arno Verschueren | Attacking Midfield | 08/04/97 | Belgium |
| Bas Kuipers | Left-Back | 17/08/94 | Netherlands |
| Sam Lammers | Centre-Forward | 30/04/97 | Netherlands |
FC Twente '65
Coach: John van den Brom
Stadium: De Grolsch Veste
Foundation: 1965